Progress in biomolecule conversion of artificial methanol
September 03, 2018 Source: Tianjin Institute of Labor and Technology, Chinese Academy of Sciences
Window._bd_share_config={ "common":{ "bdSnsKey":{ },"bdText":"","bdMini":"2","bdMiniList":false,"bdPic":"","bdStyle":" 0","bdSize":"16"},"share":{ }};with(document)0[(getElementsByTagName('head')[0]||body).appendChild(createElement('script')) .src='http://bdimg.share.baidu.com/static/api/js/share.js?v=89860593.js?cdnversion='+~(-new Date()/36e5)];China's methanol production technology is mature and the production capacity is huge. Chemical conversion can convert methanol into chemicals and fuels such as short-chain olefins in one step. However, the product types are limited, and it is particularly difficult to synthesize long-chain or complex chemicals. Biotransformation has the advantages of mild conditions, green environmental protection and rich product variety. It is a research hotspot at home and abroad through metabolic engineering to transform industrial platform microorganisms and realize biotransformation of methanol synthesis chemicals. However, the currently modified industrial platform microorganisms still use sugar as the main carbon source, and methanol only serves as an auxiliary carbon source, which limits the biotransformation of methanol.
Recently, Zheng Ping, a researcher at the Tianjin Institute of Industrial Biotechnology of the Chinese Academy of Sciences, led a team of systems and synthetic biotechnology research team and researcher Sun Jibin to lead the research team of the Center for Systems Biology to rationally design and construct methanol-dependent glutamate rods that efficiently utilize methanol. Bacillus, the conversion of methanol to the synthesis of glutamic acid. By blocking the pentose phosphate pathway, the ribulose monophosphate methanol utilization pathway was introduced, and the methanol-xylose co-utilization was used as a necessary condition for the growth of the strain, and the growth of the methanol-dependent strain was achieved. Since the methanol utilization rate is positively correlated with the cell growth rate, the growth rate of the strain and the methanol utilization rate are greatly improved by adaptive evolution. The methanol:xylose utilization ratio reaches 3.83:1, and the 13C labeling experiment indicates that the metabolites are up to 63%. The carbon comes from methanol, which becomes the main carbon source for cell growth and metabolism. The conversion of methanol to glutamic acid is also achieved by inhibiting cell wall synthesis. This study laid the foundation for the construction of an industrial platform strain that can utilize methanol as the sole carbon source to achieve efficient methanol biotransformation.
The research has been supported by the National Natural Science Foundation of China, the key deployment project of the Chinese Academy of Sciences, the key project of the International Cooperation Bureau of the Chinese Academy of Sciences, the Tianjin Youth Talents Program and the Tianjin Special Support Project. The relevant research results have been published in the journal Metabolic Engineering. Philibert Tuyishime, Ph.D. student of Tianjin Institute of Labor and Technology, and Wang Wei, assistant researcher, are the co-first authors of the paper.
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