With the advent of the era of large-scale healthy genetic testing and the development of molecular biology technology, nucleic acid is the most basic template for molecular experiments. How to choose a suitable nucleic acid extractor has become the most important thing in molecular laboratories. Many people are easy. In the choice of misunderstanding, the following examples illustrate the two most common misunderstandings:
Misunderstanding 1. The difference between nucleic acid extractor and workstation and the misunderstanding of choice <br> Many manufacturers of workstations often tell customers when selling workstations that the nucleic acid extraction device is fully automatic, and the nucleic acid extractor is semi-automatic. The workstation can be pipetting, extracting, and doing everything. It seems that as long as the sample is put in, you can get the desired nucleic acid without any tube. When the workstation is in place, it is found that the actual situation is not the same, not only not as expected. Ok, even the basic extraction of nucleic acids is difficult, so what is the reason?
The first problem, open cover problem, the cover structure of blood collection tube, saliva preservation tube, two-dimensional code frozen tube, etc. are different, the size is not the same, the manufacturer is different, how can the workstation automatically open the cover? This problem can't be solved.
The second question, barcode entry, barcode and QR code, and some manual stickers and handwritten labels, so the scan code is still manual, it is difficult to automate.
The third problem, the addition of liquid automation, workstations can achieve automatic addition of reagents, it seems to be an advantage, but adding a 96 deep well plate takes about 15 to 30 minutes, and extraction generally requires six reagents, that is, six boards About 1.5 hours, many of the extraction reagents contain high concentrations of ethanol. After a long time, the ethanol concentration will change, affecting the extraction efficiency and sensitivity. Besides, many manufacturers of extractors (such as Baitek, Baiyuan Gene, etc.) There is a pre-installed kit, no need to add liquid, the function of adding reagents automatically is a chicken rib. The configuration of the extractor plus automatic pipetting station is a routine, usually see a flight company to make up for the customer without the pre-installed kit defects.
The Nth problem, the workstation extracts nucleic acids to solve problems such as heating, shaking, mixing, magnetic attraction, etc. Each is a module. You must first determine whether the workstation you bought has this module, but also consider whether the shock can To achieve the effect of full mixing, the problem of magnetic bead recovery, whether there is a problem with the kit, whether the instrument programming is simple and whether there is an application engineer can help you optimize the extraction conditions, etc. After all, it is not only necessary to extract high-quality nucleic acids. The hardware also requires reagent matching and program optimization.
Misunderstanding 2, the difference between import and domestic, this is the biggest choice misunderstanding <br> Many people choose the nucleic acid extractor based on habitual thinking, think that imported is better than domestic; but the nucleic acid extractor belongs to the application equipment , not the basic experimental equipment, not the kind of equipment that can be used when plugged in. The nucleic acid extraction requires the overall cooperation of the instrument, the supporting kit and the application program. It is necessary for the manufacturer to select the most suitable extraction kit and program according to the sample type, which is often required. Optimize reagents and procedures, without the technical support of the manufacturer, you can't use it well. This is a good explanation for why many imported workstations and extractors are collectively armpits, and there is no reason for them; the application of imported instruments and technical support cannot keep up. Imported instrument technology requires expensive door-to-door fees, and it cannot guarantee Help the customer to optimize the experimental program (of course, the door fee must be received).
In fact, the imported nucleic acid extractor and domestically produced ratio do not have any advantages. There is a comparison table on the Internet, which is more objective:
Based on the above table, most imported nucleic acid extractors are unable to catch up with domestic manufacturers in terms of instrument operating system convenience, instrument extraction sensitivity and yield, instrument kits, and instrument upgrade and customization capabilities, and the after-sales service charges are even more serious. There is really no need to argue the superiority of imports.
From the above discussion, we should be clear that when choosing a nucleic acid extractor, it is best to conduct a full investigation or trial, so as not to fall into the passive situation of buying unusable.
Misunderstanding 1. The difference between nucleic acid extractor and workstation and the misunderstanding of choice <br> Many manufacturers of workstations often tell customers when selling workstations that the nucleic acid extraction device is fully automatic, and the nucleic acid extractor is semi-automatic. The workstation can be pipetting, extracting, and doing everything. It seems that as long as the sample is put in, you can get the desired nucleic acid without any tube. When the workstation is in place, it is found that the actual situation is not the same, not only not as expected. Ok, even the basic extraction of nucleic acids is difficult, so what is the reason?
The first problem, open cover problem, the cover structure of blood collection tube, saliva preservation tube, two-dimensional code frozen tube, etc. are different, the size is not the same, the manufacturer is different, how can the workstation automatically open the cover? This problem can't be solved.
The second question, barcode entry, barcode and QR code, and some manual stickers and handwritten labels, so the scan code is still manual, it is difficult to automate.
The third problem, the addition of liquid automation, workstations can achieve automatic addition of reagents, it seems to be an advantage, but adding a 96 deep well plate takes about 15 to 30 minutes, and extraction generally requires six reagents, that is, six boards About 1.5 hours, many of the extraction reagents contain high concentrations of ethanol. After a long time, the ethanol concentration will change, affecting the extraction efficiency and sensitivity. Besides, many manufacturers of extractors (such as Baitek, Baiyuan Gene, etc.) There is a pre-installed kit, no need to add liquid, the function of adding reagents automatically is a chicken rib. The configuration of the extractor plus automatic pipetting station is a routine, usually see a flight company to make up for the customer without the pre-installed kit defects.
The Nth problem, the workstation extracts nucleic acids to solve problems such as heating, shaking, mixing, magnetic attraction, etc. Each is a module. You must first determine whether the workstation you bought has this module, but also consider whether the shock can To achieve the effect of full mixing, the problem of magnetic bead recovery, whether there is a problem with the kit, whether the instrument programming is simple and whether there is an application engineer can help you optimize the extraction conditions, etc. After all, it is not only necessary to extract high-quality nucleic acids. The hardware also requires reagent matching and program optimization.
Misunderstanding 2, the difference between import and domestic, this is the biggest choice misunderstanding <br> Many people choose the nucleic acid extractor based on habitual thinking, think that imported is better than domestic; but the nucleic acid extractor belongs to the application equipment , not the basic experimental equipment, not the kind of equipment that can be used when plugged in. The nucleic acid extraction requires the overall cooperation of the instrument, the supporting kit and the application program. It is necessary for the manufacturer to select the most suitable extraction kit and program according to the sample type, which is often required. Optimize reagents and procedures, without the technical support of the manufacturer, you can't use it well. This is a good explanation for why many imported workstations and extractors are collectively armpits, and there is no reason for them; the application of imported instruments and technical support cannot keep up. Imported instrument technology requires expensive door-to-door fees, and it cannot guarantee Help the customer to optimize the experimental program (of course, the door fee must be received).
In fact, the imported nucleic acid extractor and domestically produced ratio do not have any advantages. There is a comparison table on the Internet, which is more objective:
Based on the above table, most imported nucleic acid extractors are unable to catch up with domestic manufacturers in terms of instrument operating system convenience, instrument extraction sensitivity and yield, instrument kits, and instrument upgrade and customization capabilities, and the after-sales service charges are even more serious. There is really no need to argue the superiority of imports.
From the above discussion, we should be clear that when choosing a nucleic acid extractor, it is best to conduct a full investigation or trial, so as not to fall into the passive situation of buying unusable.
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