First, the identification of the oestrus estrus is the key to the performance of estrus in sheep with different small goats, estrus performance is not obvious, only a few sheep can be identified by this method, the main method is the test method and vaginal examination.
1. The test method The test method is a method in which a ram is used to find ewes in order to find the estrus ewes correctly. However, when this method is adopted in rural areas, it is necessary to keep the ram and the ewes separately and to ensure that the ram's libido is to find estrus. When the ram is in the ewes group, when the ram is sniffing the ewes or hoofs to tease the ewes, the ewes will stand still, or the ewes will actively approach the rams as estrus. Use vaginal examination to judge again.
2. vaginal examination estrus vaginal mucous membrane congestion red, smooth and moist surface, there is a transparent mucus leakage, cervical mouth congestion, relaxation, opening, there is mucus outflow, you can set the heat.
Second, the sheep's sperm and semen processing technology Sheep artificial insemination technology can be divided into fresh semen artificial insemination and frozen semen artificial insemination two, it is best to sheep fresh semen artificial insemination high pregnancy rate.
1. Before extracting fine sperm, first install and disinfect the sheep with a fake vagina and apply a small amount of sterilized vaseline to the 1/3-2/3 of the inner tube to achieve the purpose of lubrication. 40-42°C warm water (150-180ml) was slowly poured from the irrigation hole into the false vaginal casing and the inner tube so that the water temperature between the outer shell and the inner tube reached 38-40°C. The water temperature may change with the season, and the winter temperature is slightly lower than the summer. high. Blow a proper amount of gas into the false vagina and adjust the pressure of the false vagina. It is appropriate to use the false vaginal entrance as “Y†or “Xâ€. Then use the ewes as mutton sheep, preferably estrus ewes. The height of the chilly sheep is higher than that of the dolphins, and it is not easy for the moor sheep to climb the ewes, and the ewes should be placed in a slightly awkward place. When the ram crawls on the ewes' backs, the ram penis is quickly introduced into the false vagina. After the ram has ejaculated, the false vagina is erected, and the collection cup is set downwards, and the collection cup is removed for semen examination. If the rams do not ejaculate several times, they should push the rams several times before picking, and if the rams do not exercise enough, the rams should be exercised.
2. The normal ejaculation amount of semen treated Dorper sheep is 1-1.5ml, milky white and cloud-like, and the density and deformity rate are observed under the microscope. All those who have sperm motility above 0.6 and semen density is above “middle†can use it. That is, 2 to 4 billion sperm per milliliter of semen can be used for production, and sperm abnormality rate must be ≤ 15% to be qualified.
Eligible semen can be used directly for insemination, but it is best to dilute according to the multiples of the dilution. If the insemination is not saved at the time, it can be diluted with VB12 or milk (used after filtration with 8 layers of gauze).
Third, the insemination technology 1. Insemination timely and accurate to a certain amount of high-quality semen into the estrus ewes estrus mouth, is the key to ensure ewes fetus. In the insemination operation, the genital area of ​​the ewes to be inseminated should be first cleaned and scrubbed with benzalkonium chloride, and then wiped clean with water or saline cotton balls, and the rear ribs of the ewes should be placed on crossbars about 70-80 cm above the ground level, or The two raised the hind legs of the sheep, and the inseminating person inserted the vagina of the ewe with an opener or an endoscope. The depth and angle were used to find the mouth of the cervix, and the inseminator was slowly inserted into the cervical os. 1.0-2.0 cm. The required amount of semen (the original fine 0.05-0.1ml) is slowly injected, and then the twitching massager is reciprocated and massaged for about 1 minute. After the opener or the endoscope is withdrawn from the vagina, the hen's thigh is slapped. Contracting the cervix helps prevent semen from draining.
2. Insemination time and number of times When the ewes begin to have estrus performance, the first insemination should be controlled after 8 to 12 hours (severe delay is appropriate for several hours), and cervical mucus characteristics can also be used as timely insemination. The mark, which is marked by a transparent mucus that becomes turbid, is best when it is finally cheese-like. Generally take 1 trial, 2 insemination, that is, the first infusion in the afternoon after the trial situation in the afternoon, the next morning and then lose 1; afternoon feelings, the next day, 1 loss in the afternoon.
3. Precautions 1 Accurately determining the estrus of the ewes is the key to guaranteeing the conception rate. It is recommended that the rams be tested for estrus.
2 Deep insemination 1.5-2.5cm can effectively increase the conception rate, and should be as deep as possible, so that the conception rate can be high.
3 In the cold winter months, the spermatozoa should be warmed up to body temperature and then placed in semen to prevent sperm freezing and shock.
4 The insemination device should be rinsed with water immediately after use and washed with distilled water for 1-2 times. After each sheep has been transfused, it should be disinfected with an alcohol swab, and then rinsed with saline twice before use.
5 Avoid all the equipment that comes into contact with sperm with water. Water can cause sperm death, so you can use saline to wash it more than twice.
6 The ewe after insemination should stay in the original Baoding position for a while and then release activities to prevent backflow of semen.
7 Insemination of virgin sheep, because of its vaginal stenosis, insemination can not reach the cervix mouth, can only be the bottom vaginal insemination, so insemination, the amount should at least double.
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