Insulation Antifreeze The temperature in early spring is still low. Solanaceous vegetables and melons and other thermophilic vegetables are vulnerable to cold injury and freezing injury. The main causes of cold damage and frost damage are the vigorous growth of plants, and the increase in water and fertilizer in human management has significantly reduced the cold resistance of plants. Solanaceous vegetables already have a large amount of growth and must cover two layers of membranes in a greenhouse. The melon vegetables are more susceptible to freezing than the eggplant vegetables. In addition to the two-layered film covering, straw bunches can be stacked around the sheds to enhance the cold air intrusion around the greenhouse; in case of strong cold air , must do a good job of antifreeze insulation measures, can temporarily warm.
Ventilation Ventilation After the beginning of spring, due to the low temperature, most vegetable farmers have less ventilation for insulation and cause high humidity in the greenhouse and high pests and diseases. In order to meet the requirements of the growing and developing environmental conditions of melons, we must coordinate the regulation of temperature and humidity in the shed, and depending on weather conditions and plant growth conditions, we should do well in opening and uncovering membrane ventilation, closing and film insulation.
The low temperature of preserved flower will affect the fertilization result of more varieties of vegetables, and it is prone to flowering and fruit falling. At present, the method of improving the fruit setting rate is to use a plant growth regulator to decorate or apply to the ovary, and to use the plant growth regulator according to the instructions, so as to prevent the concentration from being too high and the occurrence of phytotoxicity. The second is artificially assisted pollination, ie artificial flowering.
Diseases and Insect Pests Prevention and control of vegetables in the greenhouse susceptible to gray mold, disease, blight and aphids. Prevention and control of pests and diseases should adopt measures combining agricultural control and chemical control, such as reducing the humidity in the shed, paving the ditch and ditches, rationally fertilizing the water, pruning in time, removing the old leaves of the disease, and spraying the pesticide of the counterpart.
PCR ( Polymerase Chain Reaction)
It provides a way to make more copies of a portion of DNA.
We need to have PCR ingredients that include DNA portion, Buffer solution, Primer+DNA (tap polymerase), and Nucleotides during the preparation step.
In the step of PCR sequence, there are three steps in this section.
Step 1 Denaturation
The addition of heat is needed to separate the two strands of the DNA molecule.
Step 2 Annealing
Temperature, is going to be cool in this step, should allow the primers to bind to the specific segments.
Step 3 DNA Synthesis
To make more copies of the DNA.
Those three steps above keep repeating to make enough copies of the DNA fragments. It is the principle of how PCR machine works.
Superyears General 2215 RT-qPCRadopts all-in-one with an industrial art design, combining the Analytical Instrument, Large touch screen, and Computer. General 2215 RT-qPCR is equipped with the proven thermal-cooled electric technology, which can recognize the speed rate of temperature control faster and obtain high-quality testing results within 40 minutes. To ensure the sensitivity and stability of the testing a design with traditional beam path with a susceptible CMOS image sensor is used. In addition, General 2215 supports multiple PCR analysis and High-resolution Melt Curve (HRM) analysis, compatible with the mainstream reagent kit in the market. It will be the best assistant and your preferred RT-qPCR.
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