Introduction and comparison of three iQue Screener ultra high throughput suspension cells/bead screening systems

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Introduction and comparison of three iQue Screener ultra high throughput suspension cells/bead screening systems
Shanghai Dian Biotech (Shanghai) Ltd.
Intel's Intel-based iQue Screener series of ultra-high-throughput suspension cell/microbead screening system is the main product of Intellicyt. It analyzes suspended cells and microbeads by flow principle. It is a high-content screening instrument that can analyze 96-well plates. And 384-well plate, HD type can analyze 1536-well plate, with high detection speed and comprehensive analysis software.
The iQue Screener series is available in three models: iQue Screener, iQueScreener HD and the new iQue Screener PLUS.
The iQue Screener series provides the only true screening platform for screening suspended cells, microbeads, microorganisms and their mixtures. Through high-throughput flow reactions for antibody development/screening, in vitro toxicity, and phenotypic screening experiments, flow cytometry is transformed into a true screening platform for microplate analysis in minutes.
Advantages of the iQue Screener series:
(1) Faster microplate processing speed, complete the experiment in minutes instead of hours. 96-well plate 5 min, 384-well plate 15-20 min, 1536 well plate 80 min;
(2) Sample processing Mix & Read, no-wash operation;
(3) Get experimental results faster.
(1) The experimental design is miniaturized and consumes less reagents;
(2) Save precious cell samples;
(3) Saving experimental costs.
(1) A rich, diverse, single-cell based experimental result;
(2) Simultaneous detection of cells and microbeads;
(3) Secret protein analysis is available.
(1) The easiest to use operating software controlled by ForeCyt software;
(2) Intuitive, interactive visual screening interface;
(3) Annotate and visualize around the microplate.
(1) Associate massive information;
(2) operating scenario mode;
(3) Generate rich knowledge;
(4) Help to make a decision.
Applications: Antibody development/screening, in vitro toxicity, and phenotypic screening (introduced).
(1) iQue Screener:
iQue Screener is the ideal assistant for laboratories to quickly screen for suspended cells, and has access to a wealth of information about each cell and secreted protein. Especially suitable for screening precious cells or a small number of cells. For scientists who focus on revealing biological information, it uses incomprehensible techniques to conduct in-depth research and answer complex biological questions through software-supported automation and experiment-based analysis.
Main parameter characteristics:
(1) Two lasers (blue 488 nm, red 640 nm).
(2) 4 fluorescent channels (488nm: 530/30 nm, 572/28 nm, >670 nm; 640 nm: 675/30 nm, optional 780/60 nm) and 2 scattered light channels (Forward Light Scatter, Side Scatter).
(3) Fluorescence sensitivity: FITC < 150 MESF; PE < 100 MESF.
(4) Minimum particle detection diameter: 0.5 μm.
(5) Cell detection speed: up to 10,000 per second.
(6) Detection dynamic range: >7 orders of magnitude, which can simultaneously detect cells and beads.
(7) Suitable for plate type: 96-well plate, 384-well plate.
(8) Minimum sample volume in the well of the microplate: 10 μL.
(9) Minimum sample load: 1 μL.
(10) Minimum microplate analysis time: 96 min plate for 5 min, 384 well plate for 20 min.
(11) The second generation of the sampling needle, with a sensor on the top, can better locate and detect the liquid level in the hole, and can run the Between-well marker.
(2) iQue Screener PLUS
iQue Screener PLUS is designed to meet the needs of screening researchers for exploring more complex biological information, requiring more experimental choice and flexibility. It has all the features of iQue Screener for cost savings and productivity, and provides more analysis capabilities. It is equipped with 3 lasers: purple (405nm), blue (488nm) and red (640nm); or purple (405nm), blue (488nm), yellow (561nm), which can provide more detection capabilities, ie more abundant Research content. Because it uses more commercial dyes and fluorescein, it makes the experimental design more flexible and has better performance. The use of a violet laser-excited dye provides more information for cell analysis.
Main parameter characteristics:

(1) 3 lasers:

Configuration one (purple 405nm, blue 488nm, red 640nm),

Configuration two (purple 405nm, blue 488nm, yellow 561nm);

(2) 13 fluorescent channels:

Configuration one (purple 405nm: 445/45 nm, 530/30 nm, 572/28 nm, 615/24 nm, 675/30 nm, 780/60 nm; blue 488 nm: 530/30 nm, 572/28 nm, 615/24 nm, 675/30 nm, 780/60 nm; red 640 nm: 675/30 nm, 780/60 nm),

Configuration two (purple 405 nm: 445/45 nm, 530/30 nm, 586/20 nm, 615/20 nm, 660/20 nm, 780/60 nm; blue 488 nm: 530/30 nm, 615/20 nm, 660/20 nm; yellow 561 nm: 586 /20 nm, 615/20 nm, 660/20 nm, 780/60 nm).

2 Light Scatters (Side Scatter).
(3) Fluorescence sensitivity: FITC < 150 MESF; PE < 100 MESF.
(4) Minimum particle detection diameter: 0.2 μm.
(5) Cell detection speed: 35,000/sec.
(6) Detection dynamic range: >7 orders of magnitude, which can simultaneously detect cells and beads.
(7) Suitable for plate type: 96-well plate, 384-well plate.
(8) Minimum sample volume in the well of the microplate: 10 μL.
(9) Minimum sample load: 1 μL.
(10) Minimum microplate analysis time: 96 min plate for 5 min, 384 well plate for 20 min.
(11) The second generation of the sampling needle, with a sensor on the top, can better locate and detect the liquid level in the hole, and can run the Between-well marker.
(3) iQue Screener HD
iQue Screener HD has all the features that iQue Screener has to save on cost and increase productivity, and to take the experimental miniaturization to new heights. In addition to 96-well and 384-well plates, it can also analyze 1536-well plates and is the only commercially available high-content suspension cell screener that can analyze 1536-well plates.
Main parameter characteristics:
(1) Two lasers (blue 488 nm, red 640 nm).
(2) 4 fluorescent channels (488nm: 530/30 nm, 572/28 nm, >670 nm; 640 nm: 675/30 nm, optional 780/60 nm) and 2 scattered light channels (Forward Light Scatter, Side Scatter).
(3) Fluorescence sensitivity: FITC < 150 MESF; PE < 100 MESF.
(4) Minimum particle detection diameter: 0.5 μm.
(5) Cell detection speed: up to 10,000 per second.
(6) Detection dynamic range: >7 orders of magnitude, which can simultaneously detect cells and beads.
(7) Suitable for plate type: 96-well plate, 384-well plate, 1536-well plate.
(8) Minimum sample volume in the well of the microplate: 6 μL.
(9) Minimum sample load: 1 μL.
(10) Minimum microplate analysis time: 96 min plate for 5 min, 384 well plate for 20 min, and 1536 well plate for 80 min.
(11) The second generation of the sampling needle, with a sensor on the top, can better locate and detect the liquid level in the hole, and can run the Between-well marker.
Three iQue Screener applications:

Application: Suitable for all flow cytometry applications, especially: antibody development, in vitro toxicity, phenotypic screening, and tumor immunotherapy research.

(1) Antibody development includes: hybridoma screening (using cells to screen antibodies), determination of cross-reactivity of species (simultaneous reaction of various genus cells), detection of production efficiency of antibody-secreting cell lines, and screening of human IgG libraries.

(2) In vitro toxicity includes: micronucleus-induced multivariate detection, diversified apoptosis detection (simultaneous caspase pathway, Annexin V pathway, mitochondrial depolarization, cell activity, cell count and other apoptosis detection).

(3) Phenotypic screening includes: GFP expression screening of multiple cell lines, screening of compounds that induce differentiation of myeloid progenitor cells.

(4) Tumor immunotherapy includes: NK cell killing activity detection, chimeric antigen receptor T cell (CAR-T) activity detection, checkpoint inhibition (Checkpoint Inhibitor) antibody multiplex screening, simultaneous detection of compound drugs with cells and microbeads effect.

Please refer to the official website for details: http://
You can also consult Manager Liu of the Biomarkets Department: 021-58605185-822 , or pay attention to "Micro Signal: tekontech", thank you!

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