Planting method. Generally choose to order on-demand, when you order, you should do the work of spacing, generally 10 cm is the best distance, you can put about 3 seeds in each pit. Before planting, it is necessary to prepare the soil, and then the soil is thoroughly watered once. The seed before the seed is mixed with the new high-fat membrane to keep warm, moisturize and swell, improve the germination rate of the seed, and make the seedlings strong. Repel underground pests and isolate viral infections. Then sow. After sowing, gently cover the upper layer of soil and then water.
Fertilizer water. Fertilize when the seedlings are born, and fertilize when flowering and podging. Pay attention to the amount when fertilizing, in which the fertilization needs to be applied when the pod is set, and the amount is larger. If you are watering, you need to pay attention to the amount of watering, not too much. Pay attention to timely drainage during the rainy season.
Cultivate weeding. The soil permeability of the loose soil activated soil is timely. Weeding in time to build weeds to compete for plant nutrients.
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Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
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