The decline of edible fungi strains is a common phenomenon. Adopting suitable storage methods and minimizing the passage times of strains is one of the effective measures to prevent strains from declining. The storage principle of strains is to reduce their metabolism and there are many methods for storage. Two kinds of strains are mainly introduced for reference.
One is the slant cryogenic storage method, which is the simplest method of storage. It puts the growth-free, pollution-free incline tube into the refrigerator, stores it at a low temperature of 4-6°C, and then transfers it every 2-3 months. Fresh slant culture medium, and then continue to store after cultivation (to supplement the nutrients and moisture of mycelium to prevent mycelium aging).
Second, the liquid paraffin storage method This method is to store the slant test tube, in the sterile chamber injection of sterile liquid paraffin, wax injection to 1 cm above the slope is appropriate. Its purpose is to isolate bacteria from the air and reduce its metabolic activity. Then stuffed with a cork or rubber stopper, sealed with wax, upright placed in a dry place indoors, can be stored for more than one year. When using, only a small amount of mycelium is picked from the slant with an inoculation needle and transplanted on a fresh slant medium. Since the hyphae are stained with paraffin, it must be transferred to a test tube to grow well.
Enramycin Feed Additives,Enramycin Additives,Enramycin Growth Promoter,Enramycin Improve Feed Return
Shandong Shengli Bioengineering Co., Ltd , https://www.shenglipharm.com