Selection and use of ultrasonic cell disrupters

Ultrasonic cell disruption device utilizes the dispersion effect of ultrasonic waves in a liquid to cause cavitation of the liquid, thereby breaking solid particles or cell tissues in the liquid. The conventional method is to put the material to be broken into a beaker, turn on the power setting time (vibration time and intermittent time), and put the probe of the crusher into the material. During use, the ultrasonic generator circuit converts the 50/60 Hz mains into 18-21 KHz high-frequency high-voltage electric energy. Therefore, a large amount of heat is generated during the crushing process, which is generally broken under an ice bath. The ultrasonic cell disruptor consists of two parts: an ultrasonic generator and a transducer (some are equipped with a soundproof box). 1. Ultrasonic generator: Working principle: The signal generator generates a signal of a specific frequency. This specific frequency is the frequency of the transducer. The ultrasonic frequency generally used in ultrasonic equipment is 20KHz, 25KHz, 28KHz, 33KHz, 40KHz. , 60KHz. 2. Transducer assembly: The transducer assembly is mainly composed of a transducer and a horn. 3, soundproof box: can effectively reduce the noise generated during the work process, keep the laboratory quiet. The promotion of ultrasonic cell disruption instrument in China has entered a mature stage, and the application is still not popular enough! The instrument (equipment) has a wide range of applications, which is unmatched by other instruments and equipment. For this reason, the market potential of the instrument (equipment) is very large, so the number of manufacturers is increasing, which also causes the relative confusion of the ultrasonic cleaning industry and the market. It can be used in eight words to describe the "mixed fish and dragons, mixed and good"! Classify ultrasonic cell disrupters. The specific division is as follows: First, the power classification of the ultrasonic cell crusher by power is the customer's preferred indicator, which determines the quantity, size, quality and effect of the broken object. Therefore, all manufacturers also attach great importance to this indicator. Under normal circumstances, laboratories, laboratories, research institutes, drug testing institutes and other scientific research units use little power (generally below 500W); and the power used by biological companies, pharmaceutical factories, chemical companies and other production units. Most are around 500W-2000W. Due to the different product structure of each manufacturer, the method of marking power is not the same. However, according to the usual practices of users, there are generally the following types: 50W, 100W, 150W, 250W, 300W, 350W, 500W, 1000W, 2000W. Generally, the output power of the ultrasonic cell crusher can be adjusted as needed. Standard ultrasonic cell pulverizer products are rated for operation at 20 kHz. Some ultrasonic cell shredders have an auto-tuning function that allows the frequency to vary over a small range. Second, according to the probe ("tip") diameter classification processing of the volume of samples need to choose different "tip" head ultrasonic breaker. Due to the different product structure of each manufacturer, the "tip" head diameter is not the same. The general "tip" head is from a small amount of 5mm (suitable for 1ml throughput) to 25mm (suitable for 1000ml throughput), with a continuous flow probe and a throughput of up to 80 liters/hour. Replaceable "tip" heads are used in high-energy applications where wear can occur. When energy is transferred through the "tip" head, corrosion can occur where the metal surface leaves a mark. Over time, a slight erosion spot will occur where corrosion occurs. The “tip” head can be sanded with gauze or gauze unless it is damaged to a certain extent; when this happens, the “tip” head will be difficult to tune the frequency, instead it may be a long, sharp noise, Zui Cracks eventually form. To effectively process a given dose of sample, there are two main factors to consider: the "tip" head size and output power. These two factors must be matched at the same time to get good results. For small power "tip" heads, the "tip" head will not work; if too much power, the "tip" head may be damaged. Please pay attention to the required model accessories when purchasing.

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